its 4 oclock and i have nothing to do in the lab, it was 2 oclock and i had nothing to do in the lab, this never happens lol. Bored bored bored.

Ditto at work! I hate wednesdays.

Oh wait, thursdays!

Woot, tomorrow's friday! :jump:

going home soon.

T-minus 40min and counting!

I still have 3.5 hrs left~ :mad: :tear:

I still have 3.5 hrs left~ :mad: :tear:

:( :hug:

I still have 3.5 hrs left~ :mad: :tear:

yea but your boss isn't there.

i hate y'all east cost ppl -.-

I am in class right now :)

feras this is actually like a Bored@work ripoff. repost!

Yes, but being alone in the office isn't exactly thrilling. :p:

And don't give me your pity, just entertain me! :D:

Yes, but being alone in the office isn't exactly thrilling. :p:

And don't give me your pity, just entertain me! :D:

<starts a stripper pole dance>

Whoa man, you just scared everyone out of the thread! :lol:


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Kari"

at least we now know what cav does to pass the bored time in the office lol.

^ :lol: Oh man, visuals! x.x

wow. i leave for 5 minutes and crav is pole dancing. lol

wats worst is i can see his face on his avatar >.<

Photochop anyone? :evil_laug

Is it just a slow day in the work world, today!? I'm not complaining, that's for sure....

It's been slow the last couple weeks, but I'm not complaining. We're short staffed at the moment and my mom and I have been working 7 days a week for a month straight with no break. We're getting burned out.

Ah, it's threads like this that remind me how happy I am to be retired!!!!

Lab? - bored? great time to work on your lab notebook with all the little dates and initials in the corners, and the counter signatures - you can even add tabs and colors.... Or do the labels for the set up for the next day - the Devil always lies because he didn't take the time to do his prep.....

Or clean out the freezer and arrange all the bottles by alphabet, or size....check for stuff going out of date.

Also good time to get a couple of copies of Science and catch up with all the articles on Global Warming or whatever makes you laugh!

Ah, it's threads like this that remind me how happy I am to be retired!!!!

Lab? - bored? great time to work on your lab notebook with all the little dates and initials in the corners, and the counter signatures - you can even add tabs and colors.... Or do the labels for the set up for the next day - the Devil always lies because he didn't take the time to do his prep.....

Or clean out the freezer and arrange all the bottles by alphabet, or size....check for stuff going out of date.

Also good time to get a couple of copies of Science and catch up with all the articles on Global Warming or whatever makes you laugh!

the lab notebook i do on the computer, i type up my notes, i use excel and access and we use video microscopy, so its all digital, and pretty much automatic. I need tomorrows data set, query access then print.

the freezer prolly does need a cleaning out i could do that but i've mixed up a lot of my reagents in the last month since i had a few days lull time as well.

Science is where you'll find articles on global warming with actual supportable evidence, newsweek is where you'll find the popular anti-scientific global warming is fake stance. i've read the fluff i've read the actual scientific articles, im gonna go with the science after all is said and done, global warming is there and we are on a natural cyclical warming trend as well, in science its often not as polar a picture as popular media would like to make it out to be.

I wish you would send some actual Global Warming this way - there is frost on the roof this morning (7:35) and this non-scientific cold is being very hard on my tomatos! It may be warm in your area, but my propane bill is very real!

I kept my lab notes on the computer also - but we kept hard books as well - one's that we signed and so did a witness or two, to document everything - how do you keep a legal witness on a computer? Or, does today's science no longer require review and verifification?

Even stuff published in good peer reviewed Journals can be flawed. I found that out when I was doing electrophysiology on some sea slug nerves. (We used them as a model system because they gave good data, saved an animal that had a brain, and no protester was out to hug the slugs)

I had the diagrams for the prep, right from the publication, and I kept trying and trying ,to make my nerve pairs match. Slowly it occured to me that the picture was turned around and inverted. I made a transparency of it, flipped it over, and the map worked. After I hyperventillated a bit, I called up the author, who admitted that there was an error, but it helped preserve his patent-like hold on the methods. The truth was known only to his students. I have since found a number of omissions on formulas and methods, and even log books can with hold a critical element...

obviously as a woman of science you understand that a day of frost in april is probably no more than 2 standard deviations away from the average temperature to be expected, which does not constitute an outlier or a statistical significance on this one day out of hundreds in this one year out of decades. Reading anything into your local weather is unsubstantiated, you guys had a harsh winter that is lasting a little bit too long (unfortunately for your wine season), we had an extremely mild one on the east coast. How does that factor in to the total global earth equation...mildly (but which way?). I'm just saying i haven't seen any scientific evidence to disprove global warming definitively, but plenty of scientific evidence showing that industrial processes and pollution without a doubt create the conditions that can allow for greater global warming. I'll contend studying long term global climatology is extremely difficult from a logistical method with many factors involved, (time periods, earth's procession is 26,000 years; accuracy from all areas, there are hundreds of thousands of square miles on the earth each contributing to the data, and we have to analyze the atmosphere's highest levels which is obviously difficult to do with consistency, as well as studying historic data that can have been influenced by natural catastrophe....suffice it to say there is a lot of error involved). I'll remain skeptical (as a man of science i always have to, even if i read an article about how all stop signs are red ;) )but my inclination is to hypothesize that global warming is probably real.

As for how we do things here. We don't do any signing but every document is timestamped at creation and printing so we have a log and everything is kept in a printed paper copy as well in a filing cabinet. All the data is available for anyone to witness, nevertheless i have yet to discover how to add amyloid plaques to a mouse's brain ex vivo or how to trick a western blot into giving me the data i want, so im gonna stick to the pictures telling the story, and my principal investigator's review of the data to be good enough. However, I will admit, i have terrible handwriting as well, so i learned at an early age from a chem teacher in high school to type my stuff as much as possible to avoid losing marks from bad handwriting so i've been doing it ever since. Both my parents are doctors im genetically indisposed to terrible handwriting ;)

scientific articles are often only right until they are wrong, and there is obviously a desire to prove your hypotheses correctly, but i hope you realize that modern science isn't a bunch of slimy greedy types, we still believe and defend the scientific method.

Wow you really are bored!

I don't know if you were actually serious about introducing amyloid into rat brains butttt.....

I injected all sorts of things into live neurons while I was playing with electrophysiology - and many of these things were complex proteins... Is there any way that you could borrow from sea-slug electrophysiology, to inject your mouse brain? You might consider injections into cultured mouse neurons first - Meharry medical college, up in their physiology department - cultured embryonic mouse neurons and these are open and easy to view - while you get your injection systems up and running. That way you could demonstrate that your injection techniques were not harmful, of themselves, and that the changes were due only to amyloid-plaque development.

If you are rally bored you might phone that Department and just ask if anyone still has the notes on how this was done.

The University of Miami used to have an Aplysia program and they might still have someone around who knows how to construct micro injectors - good excuse to get yourself invited up there to check things out.

The USAMRICD at Aberdeen Maryland, might still have some of my old equipment, and if no one is using it, and if they haven't sent it to surplus, you might be able to borrow it for free.

oh we don't have to inject them with the proteins the little guys grow em on their own. All it took was genetically altering them to exhibit the human traited transgene for app and tau. Its quite effective the little guys certainly can and do have alzheimers.

It's weird, when I'm bored at work (programmer) I program other things and although it's not specific to my work, I still look like I'm working.

oh we don't have to inject them with the proteins the little guys grow em on their own. All it took was genetically altering them to exhibit the human traited transgene for app and tau. Its quite effective the little guys certainly can and do have alzheimers.


That is so cool!

Do they show sensitivity to aluminum? Back in the stone age, there was some thought that aluminum was replacing the calcium in the protein and this was, in part, causing the error in folding... if you deprive them of calcium and add AL to the diet, does it have any effect?

That is so cool!

Do they show sensitivity to aluminum? Back in the stone age, there was some thought that aluminum was replacing the calcium in the protein and this was, in part, causing the error in folding... if you deprive them of calcium and add AL to the diet, does it have any effect?

Interesting, currently the scope of our research is studying the affects of different inhaled anesthetics on both behavioral changes and the aggregations extracellular plaques. We don't specifically feed them a specific diet but it would be interesting to see if there is a correlation. I'm gonna read up more on the topic and see if maybe there can be another concurrent study done.

Interesting, currently the scope of our research is studying the affects of different inhaled anesthetics on both behavioral changes and the aggregations extracellular plaques. We don't specifically feed them a specific diet but it would be interesting to see if there is a correlation. I'm gonna read up more on the topic and see if maybe there can be another concurrent study done.


Be real interesting to see if ethylene had any effects. Have you done anything with it???

Back about the time nitrous oxide was found to be a gaseous hormone, we noted that ethylene production was increased in plants, and probably in animals, by exposure to certain herbicides - ethylene comes from mitochrondria, and in higher plants it acts as a signal for all sorts of processes. They used to use it as an anesthetic but there were a couple of delivery room accidents (explosions) that made safer anesthetics more popular...

We never got to do much in this direction, because a couple of plant specialists were sitting on the grants and they believed, without much evidence, that ethylene was not produced by animal cells - although their mitochrondria had a lot of commonalities... What little was out in the literature suggested that aging animal tissue produced higher levels... Because ethylene triggers so many different processes in plants, especially ones related to aging, it might be kinda fun to check out on your model...